Isolation and Serotyping of Porcine Rotaviruses and Antigenic Comparison with Other Rotavirusest
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چکیده
very effective for detecting and assaying rotaviruses. A plaque reduction neutralization test, crossprotection studies in gnotobiotic pigs, and electrophoresis of rotaviral double-stranded RNA were used for comparing viruses. Three strains produced plaques on initial isolation attempts, replicated well in cell cultures, and were antigenically very similar. We suggest that these three strains be considered porcine rotavirus serotype 1, with The Ohio State University (OSU) strain serving as the prototype. The OSU strain
منابع مشابه
Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses.
Seven rotavirus strains were isolated in cell cultures from the intestinal contents of piglets with diarrhea. MA104 cells with pancreatin in the cell culture medium was the host system of choice for virus isolation and replication. A cell culture immunofluorescence test in which MA104 cells were used in microtiter plates was very effective for detecting and assaying rotaviruses. A plaque reduct...
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